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Miltenyi Biotec
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Elabscience Biotechnology
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Journal: ACS Biomaterials Science & Engineering
Article Title: Methods and Technical Issues for Optimizing the Production of Hydrogels Containing Decellularized Wharton’s Jelly
doi: 10.1021/acsbiomaterials.5c02006
Figure Lengend Snippet: Human M1-polarized macrophages were exposed to A075G3 and A075W3 for 48 h. Positivity for CD80, a pro-inflammatory surface marker, was assessed by flow cytometry and expressed as mean fluorescence intensity (MFI) ± SD ( n = 4 for CTR, A075G3 and A075W3). * p < 0.05 (A075W3 vs CTR).
Article Snippet:
Techniques: Marker, Flow Cytometry, Fluorescence
Journal: Vaccines
Article Title: Peptide Coacervates Promote Cytosolic Delivery of STING Agonists for Cancer Immunotherapy
doi: 10.3390/vaccines14040329
Figure Lengend Snippet: Peptide and cGAMP coacervates facilitate antigen cross-presentation. ( a ) Antigen cross-presentation of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 8 h and stained with PE-SIINFEKL antibody, and then recorded by confocal laser scanning microscope. ( b – e ) Antigen cross-presentation of BMDCs or BMDCs STING KO treatment as panel a, stained with APC-SIINFEKL antibody, and then detected by flow cytometry. ( b , d ) The red dashed line serves as a reference to clearly illustrate the degree of shift across different groups. ( f – h ) CD40, CD80 and CD86 expression of BMDCs treated with OVA-SIIN (10 μM), SIIN-4A (10 μM) and cGAMP (2 μM) in indicated formulations for 24 h and detected by flow cytometry. Data are presented as mean ± SEM, n = 3 independent samples; ns, not significant, **** p < 0.0001 using one-way ANOVA with Tukey’s test.
Article Snippet: ACK Lysis Buffer (E-CK-A105), mouse bone marrow-derived dendritic cells (BMDC) induction and identification kit (XJM003), including antibodies of Elab Fluor 488-CD11c,
Techniques: Staining, Laser-Scanning Microscopy, Flow Cytometry, Expressing